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Molecular mapping of quantitative trait loci for plant growth, yield and yield related traits across three diverse locations in a doubled haploid rice population

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Abstract

A doubled haploid (DH) population of 125lines derived from IR64 × Azucena, an indica – japonica cross were grown in three different locations in India during the wet season of 1995. The parents of mapping population had diverse phenotypic values for the eleven traits observed. The DH lines exhibited considerable amount of variation for all the traits. Transgressive segregants were observed. Interval analysis with threshold LOD > 3.00 detected a total of thirty four quantitative trait loci (QTL) for eleven traits across three locations. The maximum number of twenty QTL were detected at Punjab location of North India. A total of seven QTL were identified for panicle length followed by six QTL for plant height. Eight QTL were identified on three chromosomes which were common across locations. A maximum of seven QTL were identified for panicle length with the peak LOD score of 6.01 and variance of 26.80%. The major QTL for plant height was located on Chromosome 1 with peak LOD score of 16.06 flanked by RZ730-RZ801 markers. Plant height had the maximum number of common QTL across environment at the same marker interval. One QTL was identified for grain yield per plant and four QTL for thousand grain weight. Clustering of QTL for different traits at the same marker intervals was observed for plant height, panicle exsertion, panicle number, panicle length and biomass production. This suggests that pleiotropism and or tight linkage of different traits could be the plausible reason for the congruence of several QTL. Common QTL identified across locations and environment provide an excellent opportunity for selecting stable chromosomal regions contributing to yield and yield components to develop QTL introgressed lines that can be deployed in rice breeding program.

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Authors and Affiliations

  1. Marker-Assisted Selection Laboratory, Department of Genetics and Plant Breeding, University of Agricultural Sciences, GKVK, Bangalore, 560 065, India

    Shailaja Hittalmani, H.E. Shashidhar & Prashanth G. Bagali

  2. Applied Phytologics INC, 4110N Freeway, Sacramento, CA, 95834, U.S.A

    Ning Huang

  3. Biotechnology Centre, Punjab Agricultural University, Ludhiana, 141 004, India

    J.S. Sidhu

  4. Indian Agricultural Research Institute, Pusa Road, New Delhi, 110 012, India

    V.P. Singh

  5. International Rice Research Institute, P.O. Box 933, 1099, Manila, Philippines

    G.S. Khush

Authors
  1. Shailaja Hittalmani
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  2. H.E. Shashidhar
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  3. Prashanth G. Bagali
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  4. Ning Huang
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  5. J.S. Sidhu
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  6. V.P. Singh
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  7. G.S. Khush
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Hittalmani, S., Shashidhar, H., Bagali, P.G. et al. Molecular mapping of quantitative trait loci for plant growth, yield and yield related traits across three diverse locations in a doubled haploid rice population. Euphytica 125, 207–214 (2002). https://doi.org/10.1023/A:1015890125247

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  • DOI: https://doi.org/10.1023/A:1015890125247

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